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Brucellosis Quiz
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Quiz Instructions This quiz consists of 20 multiple-choice questions . You will be awarded 5 marks for each correct answer. Question 1: Which organism was isolated by Sir David Bruce in 1887 from patients with "Mediterranean fever" or "Malta fever"? Brucella abortus Brucella suis B. melitensis Brucella ovis Question 2: What staining characteristic is unique to Brucella species, causing them to stain red as clusters in tissue smears when using the Modified Ziehl-Neelsen (MZN) method? Gram-positive Acid-fast MZN-positive Spore-forming Question 3: The striking proliferation and localization of Brucella abortus in the bovine placenta, leading to abortion, is primarily attributed to the presence of which substance in the fetal tissues? Glycogen Casein Erythritol Glucose Question 4: What is the main clinical sign of Brucellosis in cattle caused by B. abortus? Early embryonic death (1st trimester) Abortion between the seventh and eighth months of gestation Severe, fatal septicemia in adults Lumbar osteomyelitis and paralysis Question 5: Which Brucella species infection is characterized by localization in the skeleton (arthritis and osteomyelitis) in its preferred host, causing clinical signs like lameness and posterior paralysis? B. abortus B. ovis B. melitensis B. suis Question 6: What is the characteristic feature of human brucellosis (zoonosis)? Severe septic metritis Abortion during the last trimester Extensive hemorrhagic diarrhea Undulant (recurrent) fever Question 7: The inability to successfully treat brucellosis in preferred animal hosts with antibiotics is primarily due to: High antimicrobial resistance in the field Intracellular sequestration in phagocytic and reproductive cells Lack of commercially available drugs The non-motile nature of the bacteria Question 8: Which diagnostic procedure for Brucellosis carries the highest risk of laboratory-acquired infection and requires Biosafety Level 3 conditions for handling live cultures? Serum Agglutination Test (SAT) Competitive ELISA (C-ELISA) Isolation and culture of the organism Fluorescence Polarization Assay (FPA) Question 9: Which organism is known to cause false-positive results in standard Brucella serological tests due to shared antigens (O side chain LPS)? Chlamydia abortus Actinobacillus seminis Yersinia enterocolitica O:9 Listeria monocytogenes Question 10: The primary clinical manifestation of Brucella ovis infection in sheep is: Late abortion in ewes Severe generalized mastitis Systemic pyrexia (undulant fever) Epididymitis in rams Question 11: In national eradication schemes for bovine brucellosis, what is the strategy of choice for controlling the infection on infected premises? Prolonged antibiotic treatment Mass vaccination of all animals Depopulation (test and slaughter) Use of only the RB51 vaccine Question 12: Brucella species are classified as capnophilic. This means their growth is typically enhanced or required by the presence of: Serum or blood 5–10% CO2 High oxygen tension Erythritol in the media Question 13: Match the Brucella species with its typically preferred host: (a) B. abortus, (b) B. suis, (c) B. canis, (d) B. melitensis. Host Options: (i) Pigs, (ii) Dogs, (iii) Cattle, (iv) Sheep/Goats (a-i), (b-iii), (c-iv), (d-ii) (a-iii), (b-i), (c-ii), (d-iv) (a-ii), (b-iii), (c-i), (d-iv) (a-iv), (b-i), (c-iii), (d-ii) Question 14: Match the historical Brucellosis name with the associated event/organism: (a) Malta Fever, (b) Bang’s Disease, (c) Undulant Fever, (d) Traum's Identification. Associated Options: (i) Isolation of B. abortus in 1897, (ii) Caused by B. melitensis in humans, (iii) Discovery of B. suis in pig fetuses, (iv) Recurrent fever pattern in human brucellosis. (a-ii), (b-i), (c-iv), (d-iii) (a-i), (b-ii), (c-iii), (d-iv) (a-iv), (b-iii), (c-ii), (d-i) (a-iii), (b-iv), (c-i), (d-ii) Question 15: Match the Brucella species characteristic with its structural/phenotypic feature: (a) Primary cause of human mortality, (b) Always rough colony phenotype, (c) Most common complication in human infection, (d) Not considered a zoonosis. Feature Options: (i) B. ovis, (ii) Osteoarticular disease, (iii) B. melitensis, (iv) B. canis and B. ovis. (a-iii), (b-iv), (c-ii), (d-i) (a-ii), (b-i), (c-iv), (d-iii) (a-i), (b-iii), (c-ii), (d-iv) (a-iv), (b-ii), (c-iii), (d-i) Question 16: Assertion (A): The eradication of Brucella infection often necessitates the use of live attenuated vaccines, such as S19 or Rev. 1, rather than killed bacterins. Reason (R): Immunity against brucellosis is predominantly mediated by cellular immune responses, which live vaccines are more effective at stimulating. Both A and R are true, and R is the correct explanation of A. Both A and R are true, but R is NOT the correct explanation of A. A is true, but R is false. A is false, but R is true. Question 17: Assertion (A): Ingestion of raw milk contaminated with B. melitensis is a major public health concern, especially leading to severe cases of undulant fever in humans. Reason (R): Abortion is a prominent clinical feature in pregnant women infected with Brucella melitensis. Both A and R are true, and R is the correct explanation of A. Both A and R are true, but R is NOT the correct explanation of A. A is true, but R is false. A is false, but R is true. Question 18: Assertion (A): The Serologic Agglutination Test (SAT) is universally sufficient to confirm a Brucella infection status in cattle herds. Reason (R): Serological tests for Brucella often produce false-positive results due to cross-reactivity with the LPS O side chain of organisms like Yersinia enterocolitica O:9. Both A and R are true, and R is the correct explanation of A. Both A and R are true, but R is NOT the correct explanation of A. A is true, but R is false. A is false, but R is true. Question 19: Assertion (A): Brucella organisms have a striking affinity for the reproductive organs, placenta, and fetal fluids in their natural hosts. Reason (R): The organism survives in these tissues because they contain erythritol, a sugar that promotes Brucella growth. Both A and R are true, and R is the correct explanation of A. Both A and R are true, but R is NOT the correct explanation of A. A is true, but R is false. A is false, but R is true. Question 20: Assertion (A): Bulls infected with B. abortus are not considered to play a significant role in the natural transmission of the disease within cattle herds. Reason (R): The typical transmission route for B. abortus is primarily lateral via the ingestion of infective uterine discharge, aborted fetuses, or placenta, rather than venereal transmission. Both A and R are true, and R is the correct explanation of A. Both A and R are true, but R is NOT the correct explanation of A. A is true, but R is false. A is false, but R is true.
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